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intercept (tbs) blocking buffer  (LI-COR)


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    Structured Review

    LI-COR intercept (tbs) blocking buffer
    Intercept (Tbs) Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 7502 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/intercept+tbs+blocking+buffer/Intercept+(TBS)+Blocking+Buffer/custom%40927-60001%4042389555
    Average 98 stars, based on 7502 article reviews
    intercept (tbs) blocking buffer - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    other:

    Article Title: Cellular and molecular basis of PfCoronin function in artemisinin resistance in Plasmodium falciparum.
    Article Snippet: Separated proteins were transferred to a nitrocellulose membrane (Life Technologies Cat. No. IB3010-31) using an iBlot system (Life Technologies).

    Membrane:

    Article Title: Unbiased proteomics following inflammasome activation identifies caspase targets in primary intestinal epithelial cells
    Article Snippet: The Trans-Blot Turbo Transfer System (Bio-Rad) was used to transfer to a 0.45 μm pore size PVDF membrane (Bio-Rad). .. The PVDF membrane was blocked for 1 hour at room temperature using Intercept (TBS) Blocking Buffer (927-60001, LI-COR). ..

    Article Title: Prolactin, cortisol, and extracellular osmolality regulate cftr , ostf1 , and sgk1 in tilapia ionocytes
    Article Snippet: .. After transfer, the membrane was washed in 1X PBS and incubated in Intercept (TBS) Blocking Buffer (Li-Cor) for 1 h at room temperature. .. Primary antibody incubations with polyclonal rabbit anti-Sgk (1:1000; S5188, MilliporeSigma, Burlington, MA) and monoclonal mouse anti-β actin (1:1000; 08691001, MPBio, Santa Ana, CA) were carried out overnight at 4 °C.

    Article Title: Role of Collection Media on the Biological Activity of Extracellular Vesicles From hTERT‐Immortalised Mesenchymal Stromal Cells
    Article Snippet: The proteins from the gel were transferred to a Trans‐Blot Turbo Midi 0.2 μm PVDF Transfer Packs (Bio‐Rad) nitrocellulose membrane using the Mix MW function of the Trans‐Blot Turbo Transfer System. .. The membrane was stained with Ponceau S solution (Sigma–Aldrich) and then blocked with 50% Intercept (TBS) Blocking Buffer (Licor) in TBS (Thermo Fisher Scientific). .. Following blocking, the membrane was incubated overnight at 4°C with primary antibodies in v/v 50%/50% Intercept (TBS) Blocking Buffer (Licor)/TBS (Thermo Fisher Scientific) supplemented with 0.1% Tween‐20 (Sigma–Aldrich).

    Article Title: Alzheimer's disease risk protein SorLA regulates ER homeostasis and lipid metabolism in human microglia, with conserved effects in neurons.
    Article Snippet: Microglial dysfunction is a hallmark of Alzheimer’s disease (AD), yet the molecular mechanisms driving these impairments remain poorly defined.. Genetic studies implicate several AD-associated genes in regulating microglial activity, including SORL1, which encodes the sorting receptor SorLA.. Although SorLA is highly expressed in microglia, its functional role in cellular homeostasis has remained unclear.

    Blocking Assay:

    Article Title: Unbiased proteomics following inflammasome activation identifies caspase targets in primary intestinal epithelial cells
    Article Snippet: The Trans-Blot Turbo Transfer System (Bio-Rad) was used to transfer to a 0.45 μm pore size PVDF membrane (Bio-Rad). .. The PVDF membrane was blocked for 1 hour at room temperature using Intercept (TBS) Blocking Buffer (927-60001, LI-COR). ..

    Article Title: CPT1A loss promotes lung metastasis in immune-competent mice via a mechanism of mtDNA release and chronic activation of STING pathway
    Article Snippet: Cells were lysed in RIPA buffer (Sigma-Aldrich #R0278) supplemented with protease and phosphatase inhibitors for 30 min on ice, followed by centrifugation to remove debris. .. Membranes were blocked in INTERCEPT TBS Blocking Buffer (LI-COR Biosciences #927-60010) and incubated with the following primary antibodies at 1:1000, unless indicated: Cas9 (Cell Signaling Technology (CST) #14697), CPT1A (Abcam #ab128568 or Abcam #ab234111 or Proteintech #15184-1-AP), phospho-STING (Ser365, CST #72971), STING (CST #50494), phospho-TBK1 (Ser172, CST #5483), TBK1 (CST #3504), phoshpo-IKKε (Ser172, Invitrogen #PA5-105888), IKKε (CST #3416), phospho-p65 (Ser536, CST #3033), p65 (CST #8242), CypD (Thermo Fisher #455900), cGAS (CST #31659), VDAC (XXX), Vinculin (CST #13901), or β-Tubulin (Sigma #T4026). .. Protein concentrations were determined using the DC Protein Assay Kit II (Bio-Rad #5000112).

    Article Title: Prolactin, cortisol, and extracellular osmolality regulate cftr , ostf1 , and sgk1 in tilapia ionocytes
    Article Snippet: .. After transfer, the membrane was washed in 1X PBS and incubated in Intercept (TBS) Blocking Buffer (Li-Cor) for 1 h at room temperature. .. Primary antibody incubations with polyclonal rabbit anti-Sgk (1:1000; S5188, MilliporeSigma, Burlington, MA) and monoclonal mouse anti-β actin (1:1000; 08691001, MPBio, Santa Ana, CA) were carried out overnight at 4 °C.

    Article Title: Role of Collection Media on the Biological Activity of Extracellular Vesicles From hTERT‐Immortalised Mesenchymal Stromal Cells
    Article Snippet: The proteins from the gel were transferred to a Trans‐Blot Turbo Midi 0.2 μm PVDF Transfer Packs (Bio‐Rad) nitrocellulose membrane using the Mix MW function of the Trans‐Blot Turbo Transfer System. .. The membrane was stained with Ponceau S solution (Sigma–Aldrich) and then blocked with 50% Intercept (TBS) Blocking Buffer (Licor) in TBS (Thermo Fisher Scientific). .. Following blocking, the membrane was incubated overnight at 4°C with primary antibodies in v/v 50%/50% Intercept (TBS) Blocking Buffer (Licor)/TBS (Thermo Fisher Scientific) supplemented with 0.1% Tween‐20 (Sigma–Aldrich).

    Article Title: TCF25 emerges as a cytoplasmic regulator of GPRASP2 stability
    Article Snippet: Proteins were separated using 4–16% Tris–glycine SDS–PAGE gels and transferred onto Immobilon®-FL PVDF membranes (Merck). .. Membranes were blocked using Intercept®-TBS blocking buffer (LI-COR Biosciences). ..

    Article Title: Alzheimer's disease risk protein SorLA regulates ER homeostasis and lipid metabolism in human microglia, with conserved effects in neurons.
    Article Snippet: Microglial dysfunction is a hallmark of Alzheimer’s disease (AD), yet the molecular mechanisms driving these impairments remain poorly defined.. Genetic studies implicate several AD-associated genes in regulating microglial activity, including SORL1, which encodes the sorting receptor SorLA.. Although SorLA is highly expressed in microglia, its functional role in cellular homeostasis has remained unclear.

    Article Title: Neurokinin-1 receptor activation protects against cardiac fibrosis, inflammation and diastolic dysfunction in type 2 diabetic mice.
    Article Snippet: .. The membranes were then blocked in Intercept (TBS) blocking buffer (Li-Cor) for 1 h at room temperature before undergoing overnight incubation with primary antibodies: rabbit anti mouse p-AKT (1:1000, Cell Signaling Technology Cat# 4058, RRID:AB_331168), rabbit anti mouse Smad7 (1:1000, Abcam Cat# ab272928, RRID:AB_2924382), rabbit anti mouse p-P65 (1:1000, Cell Signaling Technology Cat# 3033, RRID:AB_331284) and rabbit anti mouse GAPDH (1:1000, Sigma-Aldrich Cat# G9545, RRID:AB_ 796208). .. Once secondary antibodies (1:10000, goat anti rabbit 700 or 800, Li-Cor) were applied for 1 h, the membranes were imaged using a Li-Cor Odyssey CL-X imager (Li-Cor) with the appropriate laser channel.

    Incubation:

    Article Title: CPT1A loss promotes lung metastasis in immune-competent mice via a mechanism of mtDNA release and chronic activation of STING pathway
    Article Snippet: Cells were lysed in RIPA buffer (Sigma-Aldrich #R0278) supplemented with protease and phosphatase inhibitors for 30 min on ice, followed by centrifugation to remove debris. .. Membranes were blocked in INTERCEPT TBS Blocking Buffer (LI-COR Biosciences #927-60010) and incubated with the following primary antibodies at 1:1000, unless indicated: Cas9 (Cell Signaling Technology (CST) #14697), CPT1A (Abcam #ab128568 or Abcam #ab234111 or Proteintech #15184-1-AP), phospho-STING (Ser365, CST #72971), STING (CST #50494), phospho-TBK1 (Ser172, CST #5483), TBK1 (CST #3504), phoshpo-IKKε (Ser172, Invitrogen #PA5-105888), IKKε (CST #3416), phospho-p65 (Ser536, CST #3033), p65 (CST #8242), CypD (Thermo Fisher #455900), cGAS (CST #31659), VDAC (XXX), Vinculin (CST #13901), or β-Tubulin (Sigma #T4026). .. Protein concentrations were determined using the DC Protein Assay Kit II (Bio-Rad #5000112).

    Article Title: Prolactin, cortisol, and extracellular osmolality regulate cftr , ostf1 , and sgk1 in tilapia ionocytes
    Article Snippet: .. After transfer, the membrane was washed in 1X PBS and incubated in Intercept (TBS) Blocking Buffer (Li-Cor) for 1 h at room temperature. .. Primary antibody incubations with polyclonal rabbit anti-Sgk (1:1000; S5188, MilliporeSigma, Burlington, MA) and monoclonal mouse anti-β actin (1:1000; 08691001, MPBio, Santa Ana, CA) were carried out overnight at 4 °C.

    Article Title: Alzheimer's disease risk protein SorLA regulates ER homeostasis and lipid metabolism in human microglia, with conserved effects in neurons.
    Article Snippet: Microglial dysfunction is a hallmark of Alzheimer’s disease (AD), yet the molecular mechanisms driving these impairments remain poorly defined.. Genetic studies implicate several AD-associated genes in regulating microglial activity, including SORL1, which encodes the sorting receptor SorLA.. Although SorLA is highly expressed in microglia, its functional role in cellular homeostasis has remained unclear.

    Article Title: Neurokinin-1 receptor activation protects against cardiac fibrosis, inflammation and diastolic dysfunction in type 2 diabetic mice.
    Article Snippet: .. The membranes were then blocked in Intercept (TBS) blocking buffer (Li-Cor) for 1 h at room temperature before undergoing overnight incubation with primary antibodies: rabbit anti mouse p-AKT (1:1000, Cell Signaling Technology Cat# 4058, RRID:AB_331168), rabbit anti mouse Smad7 (1:1000, Abcam Cat# ab272928, RRID:AB_2924382), rabbit anti mouse p-P65 (1:1000, Cell Signaling Technology Cat# 3033, RRID:AB_331284) and rabbit anti mouse GAPDH (1:1000, Sigma-Aldrich Cat# G9545, RRID:AB_ 796208). .. Once secondary antibodies (1:10000, goat anti rabbit 700 or 800, Li-Cor) were applied for 1 h, the membranes were imaged using a Li-Cor Odyssey CL-X imager (Li-Cor) with the appropriate laser channel.

    Staining:

    Article Title: Role of Collection Media on the Biological Activity of Extracellular Vesicles From hTERT‐Immortalised Mesenchymal Stromal Cells
    Article Snippet: The proteins from the gel were transferred to a Trans‐Blot Turbo Midi 0.2 μm PVDF Transfer Packs (Bio‐Rad) nitrocellulose membrane using the Mix MW function of the Trans‐Blot Turbo Transfer System. .. The membrane was stained with Ponceau S solution (Sigma–Aldrich) and then blocked with 50% Intercept (TBS) Blocking Buffer (Licor) in TBS (Thermo Fisher Scientific). .. Following blocking, the membrane was incubated overnight at 4°C with primary antibodies in v/v 50%/50% Intercept (TBS) Blocking Buffer (Licor)/TBS (Thermo Fisher Scientific) supplemented with 0.1% Tween‐20 (Sigma–Aldrich).



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